Product Name :
PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68]
Predicted band size :
113 kDa
Observed band size :
113 kDa
Synonyms:
ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody 展开
Function :
The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes.Formula of 1022159-15-4 Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1).Formula of 1,10-Phenanthrolin-5-amine After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG).PMID:33392958 PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within N-terminal human PARP1.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, FC
Coupling :
unconjugated
Clone number:
SU03-68
RRID:
AB_3069814
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 113 kDa
Positive control :
A549 cell lysate, Jurkat cell lysate, Hela cell lysate, HeLa, human spleen tissue, mouse large intestine tissue, human breast carcinoma tissue, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate.